File:13 Hegasy CRISPR pre crRNA Wiki E CCBYSA.png
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[edit]Description13 Hegasy CRISPR pre crRNA Wiki E CCBYSA.png |
English: Transcripts of the CRISPR Genetic Locus and Maturation of pre-crRNA
1. The CRISPR locus of S. pyogenes consists of a cas operon, a CRISPR array, and genes coding for trans-activating crRNA (tracrRNA). tracrRNA, Cas9 RNA and pre-crRNA are transcribed from the CRISPR genetic locus. tracrRNA then forms a tertiary structure with three loops and Cas9 RNA is further translated in protein. 2. Because pre-crRNA is synthesized as a continuous stretch, a maturation process follows. In this process, tracrRNAs pair with repeat sequences of pre-crRNA. Cas9 protein is recruited to form a ternary complex with the tracrRNA and pre-crRNA. Single complexes are then released through cleavage by RNAse III enzymes. 3. The surveillance and interference complex consists of Cas9 and a duplex of tracrRNA and crRNA. crRNA contains the spacer sequence for comparison with DNA, and a sequence to form a duplex with tracrRNA. |
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Author | Guido4 |
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current | 14:43, 1 November 2017 | 2,074 × 1,943 (272 KB) | Guido4 (talk | contribs) | User created page with UploadWizard |
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1 November 2017
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